Microchip separation and electrochemical detection of amino acids and peptides following precolumn derivatization with naphthalene-2,3-dicarboxyaldehyde
An integrated microfluidic device is used to derivatize, separate, and amperometrically detect amino acids and peptides in the presence of naphthalene-2,3-dicarboxyaldehyde (NDA). The integrated system offers a rapid (4 min) simultaneous measurements of 5 amino acids (Arg, Lys, Gly, Cys, PhenA) down to the 3.2 muM level in connection to a precolumn reaction chamber, an …
